ATSbio丨ATSbio 192-IgG-SAP说明书

脑室内注射192 IgG SAP（192 Saporin）导致大鼠LNGFR（p75NTR）阳性细胞几乎完全消除。192 IgG SAP针对仅在胆碱能基底前脑（CBF）神经元上高水平表达的细胞表面抗原。抗原p75NTR不在邻近的非胆碱能神经元上表达。192-IgG-SAP特异性地消除了基底前脑、中间隔、布罗卡斜带、Meynert基底核和小脑浦肯野神经元的胆碱能神经元。它为研究人员提供了一种强大的损伤工具——比化学、外科或电解损伤更具体有效。胆碱能前脑神经元的永久性和选择性移除是研究行为、神经元损失（如阿尔茨海默病）、其他系统对损失的反应、替代治疗以及药物效应和依赖性的重要动物模型。

Advanced Targeting Systems/ATSbio 192 IgG SAP是小鼠抗大鼠p75NTR单克隆抗体和核糖体失活蛋白saporin的化学缀合物。它特别地消除了表达p75NTR的细胞，也被称为低亲和力神经生长因子受体。

Advanced Targeting Systems/ATSbio 其它高引文数量研究：

IT-14 CTB-SAP 靶向表达GM1受体的细胞

IT-16 mu p75-SAP 靶向表达小鼠p75NTR的细胞

IT-03 Anti-DBH-SAP 靶向表达大鼠多巴胺β羟化酶的细胞

IT-10 IB4-SAP 靶向表达α-D-吡喃半乳糖苷残基的细胞

Advanced Targeting Systems/ATSbio 192 IgG SAP相关文献：

Neurotoxic effects, mechanisms, and outcome of 192 IgG-Saporin lesions.

Petrosini L, De Bartolo P, Cutuli D (2021) Neurotoxic effects, mechanisms, and outcome of 192 IgG-Saporin lesions. RM Kostrzewa (Ed.): Handbook of Neurotoxicity . Springer, Cham doi: 10.1007/978-3-030-71519-9_79-1

Summary: 192-IgG-saporin selectively destroys basal forebrain cholinergic neurons that provide cholinergic input to the hippocampus, entire cortical mantle, amygdala, and olfactory bulb. Immunotoxic lesions by 192-IgG-saporin represent a valid animal model of Alzheimer’s disease, given the degeneration of basal cholinergic system present in this pathology. The selective lesioning of cholinergic innervation by means of 192-IgG-saporin (injected i.p. or i.c.v.) is able to interfere with experience-dependent plasticity.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Pain sensitivity following loss of cholinergic basal forebrain (CBF) neurons in the rat.

Vierck C, Yezierski R, Wiley R (2016) Pain sensitivity following loss of cholinergic basal forebrain (CBF) neurons in the rat. Neuroscience 319:23-34. doi: 10.1016/j.neuroscience.2016.01.038

Objective: There is a large amount of research on the involvement of cholinergic mechanisms on spinal transmission of pain signals, indicating that cholinergic agonists can attenuate this kind of pain. In contrast, some studies have shown affective reactions to pain are suppressed by cholinergic antagonists. The authors investigated the disagreement between reflexive and affective reactions.

Summary: Lesioned rats displayed decreased escape from thermal stimulation, as well as loss of the normal hyperalgesic effect of sound stress. Results indicate that the basal forebrain cholinergic system plays a role in central processing of pain.

Usage: Administration of 192-IgG-SAP with a 4-μg injection into the left lateral ventricle of rats. Animals were tested in temperature escape and sound stress models.

Related Products: 192-IgG-SAP (Cat. #IT-01)