中英文说明丨InVivoMAb 抗小鼠 IL-4介绍
The 11B11 monoclonal antibody reacts with mouse IL-4 (interleukin-4) a multifunctional 14 kDa cytokine. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4Rα. Upon receptor binding IL-4 stimulates activated B and T lymphocyte proliferation, and the differentiation of B cells into plasma cells. It also induces B cell class switching to IgE, and up-regulates MHC class II production while decreasing the production of Th1 cells, macrophages, IFNγ, and dendritic cell IL-12. Like other Th2 associated cytokines, IL-4 is involved in the airway inflammation observed in the lungs of patients with allergic asthma. The 11B11 monoclonal antibody has been shown to neutralize the bioactivity of natural or recombinant IL-4.
Isotype Rat IgG1, κ
Recommended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Partially purified native mouse IL-4
Reported Applications in vivo IL-4 neutralization
in vitro IL-4 neutralization
in vivo IL-4 receptor stimulation (as a complex with IL-4)
Flow cytometry
Western blot
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin 2EU/mg ( 0.002EU/μg)
Determined by LAL gel clotting assay
Purity 95%
Determined by SDS-PAGE
Sterility 0.2 μM filtered
Production Purified from tissue culture supernatant in an animal free facility
Purification Protein G
RRID AB_1107707
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
11B11单克隆抗体与小鼠IL-4(白细胞介素-4)反应,这是一种多功能14kDa细胞因子。IL-4主要由活化的Th2细胞和NK细胞表达,在较低水平上由肥大细胞和嗜碱性粒细胞表达。IL-4通过IL-4Rα发出信号。受体结合后,IL-4刺激活化的B和T淋巴细胞增殖,以及B细胞分化为浆细胞。它还诱导B细胞类切换到IgE,并上调MHC II类的产生,同时减少Th1细胞,巨噬细胞,IFNγ和树突状细胞IL-12的产生。与其他Th2相关细胞因子一样,IL-4参与过敏性哮喘患者肺部观察到的气道炎症。11B11单克隆抗体已被证明可以中和天然或重组IL-4的生物活性。
InVivoMAb 抗小鼠 IL-4#BE0045基本参数:
同种型 大鼠IgG1, κ
推荐的同种型对照 体内MAb大鼠IgG1同种型对照,抗辣根过氧化物酶
推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液
免疫原 部分纯化的天然小鼠IL-4
报告的应用程序 体内IL-4体外中和IL-4体内中
和
IL-4受体刺激(与IL-4复合)
流式细胞术
蛋白质印迹
配方 PBS,pH 7.0
不含稳定剂或防腐剂
内毒素 2EU/mg ( 0.002EU/μg)
通过LAL凝胶凝血测定法测定
纯度 95%
由 SDS-PAGE 决定
无 菌 0.2 μM 过滤
生产 从无动物设施中的组织培养上清液中纯化
纯化 蛋白 G
里德 AB_1107707
分子量 150 千达
存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。
InVivoMAb抗小鼠IL-4部分文献引用:
in vitro IL-4 neutralization
Hill, E. V., et al. (2015). Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter Eur J Immunol 45(4): 1103-1115.
in vitro IL-4 neutralization
Choi, Y. S., et al. (2015). LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6 Nat Immunol 16(9): 980-990.
in vitro IL-4 neutralization
Kim, Y. U., et al. (2015). Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells PLoS One 10(3): e0120294.
in vitro IL-4 neutralization
Hou, L., et al. (2015). The protease cathepsin L regulates Th17 cell differentiation J Autoimmun. S 0896-8411(15): 30024-X
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