背景：Plants typically exhibit the purple phenomenon as a result of an increase in flavonoids and anthocyanins. A new tea germplasm 'P113' was recently selected from Camellia tachangensis, which is purple in tender shoots.
结果：In the present study, integrated transcriptome and metabolome were used to analyze the flavonoid metabolite components and the genes involved in flavonoid biosynthesis in 'P113'. A total of 86 flavonoid metabolites were identified, including 70 significantly differential metabolites (p < 0.05) and they were enriched to the three metabolic pathways of ko00941, ko00942 and ko00944 through KEGG pathway analysis. A total of 136 flavonoid involved genes were obtained from transcriptomic study, of which 53 were significantly differentially expressed in developmental shoots. The correlation between metabolite profiling and transcriptome, transcriptome and protein interactions suggested that transcription factor MYB12 and glycosyltransferase UGT78D2 had a good facilitation on purple tender shoots.结论：The metabolic pathways and potential genes that underlie the coloration of the shoots in 'P113' are clarified in this study. It also lays the groundwork for identifying potential genes involved in color variation and offers a theoretical framework for the creation and use of distinctive genetic resources and the breeding of new cultivars. 由于类黄酮和花青素的增加，植物通常呈现紫色现象。最近从大厂茶（Camellia tachangensis）中选出了一个新的茶树种质'P113’，其嫩枝呈紫色。在本研究中，使用整合转录组和代谢组分析了“P113”中类黄酮代谢产物成分和类黄酮生物合成相关基因。共鉴定了86种黄酮类代谢产物，包括70种显著差异的代谢产物（p<0.05），通过KEGG途径分析，它们被富集到三种代谢途径：ko00941、ko00942和ko00944。从转录组研究中共获得136个黄酮类相关基因，其中53个在发育嫩梢中显著差异表达。代谢产物谱与转录组、转录组和蛋白质相互作用之间的相关性表明，转录因子MYB12和糖基转移酶UGT78D2对紫色嫩梢有良好的促进作用。这项研究阐明了“P113”中嫩枝着色的代谢途径和潜在基因。它还为识别与颜色变异有关的潜在基因奠定了基础，并为创造和使用独特的遗传资源以及培育新品种提供了理论框架。
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紫茶品种“P113”中类黄酮生物合成解析
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